Motivation is a cognitive decision-making process through which purposeful behavior is initiated, guided, and maintained. Given the importance and application of employee motivation in organizations, its concepts and components are still not well understood. Accordingly, the aim of the present study was to design and validate a framework of human capital motivational concepts based on the Holy Quran. In this study, a sequential exploratory mixed design of the classification type was used. In the first part of the research (qualitative part), qualitative data were collected and analyzed based on the analytical cycle process method, and in order to validate the qualitative results, validity and reliability techniques were used, and then in the second part of the research (quantitative), quantitative data were collected and analyzed based on the qualitative results obtained. In the quantitative part, based on the framework developed in the first part of the research, a scale was designed and implemented in a wide range of state, military, and non-governmental organizations (n=324) in Fars province. The validity of the developed motivational framework was assessed and confirmed using confirmatory factor analysis. The results of the quantitative section were then used to better explain the qualitative results. The results showed that the developed framework has the necessary validity, usefulness, and desirable implementability. The results also indicated that all three dimensions of motivational orientations, principles, and methods in the aforementioned framework and their related components have the necessary adequacy and validity.